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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which leads to chromosome instability, and therefore could add to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital purpose in advertising cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two extra splice variants were reported but in-depth nucleotide sequence details isn't nonetheless obtainable.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide usually consists of the epitope recognized by the antibody.
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This manual outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best benefits are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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